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Notch2 interacted with Wnt2 and β-catenin in POF mice. ( A ) qPCR was used to detect Wnt-2 and β-catenin mRNA expressions in the ovarian tissues. ( B ) Immunohistochemistry was applied to measure Notch2 protein expression in the ovarian tissues. Magnification×200, scale bar = 100 μm. ( C ) Western blot analysis was utilized to test Notch2 pathway-related protein expressions (Notch2, <t>Jag1</t> and Hes2) and Wnt2/β-catenin pathway-related protein expressions (Wnt2, β-catenin, Axin2 and LEF1) in the ovaries. ( D ) Co-immunoprecipitation (Co-IP) was conducted to further verify the protein-protein interactions between Notch2 and Wnt2. P < 0.05 and P < 0.01 vs. Control; # P < 0.05 and ## P < 0.01 vs. oe-NC; @ P < 0.05 and @@ P < 0.01 vs. oe-Notch2. Results were presented as mean ± SD
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Notch2 interacted with Wnt2 and β-catenin in POF mice. ( A ) qPCR was used to detect Wnt-2 and β-catenin mRNA expressions in the ovarian tissues. ( B ) Immunohistochemistry was applied to measure Notch2 protein expression in the ovarian tissues. Magnification×200, scale bar = 100 μm. ( C ) Western blot analysis was utilized to test Notch2 pathway-related protein expressions (Notch2, Jag1 and Hes2) and Wnt2/β-catenin pathway-related protein expressions (Wnt2, β-catenin, Axin2 and LEF1) in the ovaries. ( D ) Co-immunoprecipitation (Co-IP) was conducted to further verify the protein-protein interactions between Notch2 and Wnt2. P < 0.05 and P < 0.01 vs. Control; # P < 0.05 and ## P < 0.01 vs. oe-NC; @ P < 0.05 and @@ P < 0.01 vs. oe-Notch2. Results were presented as mean ± SD

Journal: Journal of Ovarian Research

Article Title: Notch2 improves granulosa cell functions in premature ovarian failure by activating the Wnt2/β-catenin pathway

doi: 10.1186/s13048-025-01745-9

Figure Lengend Snippet: Notch2 interacted with Wnt2 and β-catenin in POF mice. ( A ) qPCR was used to detect Wnt-2 and β-catenin mRNA expressions in the ovarian tissues. ( B ) Immunohistochemistry was applied to measure Notch2 protein expression in the ovarian tissues. Magnification×200, scale bar = 100 μm. ( C ) Western blot analysis was utilized to test Notch2 pathway-related protein expressions (Notch2, Jag1 and Hes2) and Wnt2/β-catenin pathway-related protein expressions (Wnt2, β-catenin, Axin2 and LEF1) in the ovaries. ( D ) Co-immunoprecipitation (Co-IP) was conducted to further verify the protein-protein interactions between Notch2 and Wnt2. P < 0.05 and P < 0.01 vs. Control; # P < 0.05 and ## P < 0.01 vs. oe-NC; @ P < 0.05 and @@ P < 0.01 vs. oe-Notch2. Results were presented as mean ± SD

Article Snippet: Jag1 , BIOSS , bs-1448R , 1:1000.

Techniques: Immunohistochemistry, Expressing, Western Blot, Immunoprecipitation, Co-Immunoprecipitation Assay, Protein-Protein interactions, Control

Notch2 acted on the Wnt2/β-catenin pathway in POF cells. ( A ) qPCR analysis for the expressions Wnt2 and β-catenin mRNAs in KNG cells. ( B ) Western blot analysis for the expressions of Notch2, Jag1, Hes2 Wnt2, β-catenin, Axin2 and LEF1 proteins in KNG cells. P < 0.05 and P < 0.01 vs. Control; * P < 0.05 and ** P < 0.01 vs. Model; # P < 0.05 and ## P < 0.01 vs. sh-NC; @ P < 0.05 and @@ P < 0.01 vs. sh-Notch2. Results were presented as mean ± SD

Journal: Journal of Ovarian Research

Article Title: Notch2 improves granulosa cell functions in premature ovarian failure by activating the Wnt2/β-catenin pathway

doi: 10.1186/s13048-025-01745-9

Figure Lengend Snippet: Notch2 acted on the Wnt2/β-catenin pathway in POF cells. ( A ) qPCR analysis for the expressions Wnt2 and β-catenin mRNAs in KNG cells. ( B ) Western blot analysis for the expressions of Notch2, Jag1, Hes2 Wnt2, β-catenin, Axin2 and LEF1 proteins in KNG cells. P < 0.05 and P < 0.01 vs. Control; * P < 0.05 and ** P < 0.01 vs. Model; # P < 0.05 and ## P < 0.01 vs. sh-NC; @ P < 0.05 and @@ P < 0.01 vs. sh-Notch2. Results were presented as mean ± SD

Article Snippet: Jag1 , BIOSS , bs-1448R , 1:1000.

Techniques: Western Blot, Control

βcatenin knockdown inhibited Wnt2/β-catenin pathway in POF cells treated with sh-Notch2 and SKL2001 ( A ) qPCR analysis for the expressions Wnt2 and β-catenin mRNAs in KNG cells. ( B ) Western blot analysis for the expressions of Notch2, Jag1, Hes2 Wnt2, β-catenin, Axin2 and LEF1 proteins in KNG cells. # P < 0.05 and ## P < 0.01 vs. sh-Notch2 + SKL2001 + sh-NC. Results were presented as mean ± SD

Journal: Journal of Ovarian Research

Article Title: Notch2 improves granulosa cell functions in premature ovarian failure by activating the Wnt2/β-catenin pathway

doi: 10.1186/s13048-025-01745-9

Figure Lengend Snippet: βcatenin knockdown inhibited Wnt2/β-catenin pathway in POF cells treated with sh-Notch2 and SKL2001 ( A ) qPCR analysis for the expressions Wnt2 and β-catenin mRNAs in KNG cells. ( B ) Western blot analysis for the expressions of Notch2, Jag1, Hes2 Wnt2, β-catenin, Axin2 and LEF1 proteins in KNG cells. # P < 0.05 and ## P < 0.01 vs. sh-Notch2 + SKL2001 + sh-NC. Results were presented as mean ± SD

Article Snippet: Jag1 , BIOSS , bs-1448R , 1:1000.

Techniques: Knockdown, Western Blot